44k salmonid oligonucleotide microarray platform (Agilent technologies)
Structured Review

44k Salmonid Oligonucleotide Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Gill and Liver Transcript Expression Changes Associated With Gill Damage in Atlantic Salmon ( Salmo salar )"
Article Title: Gill and Liver Transcript Expression Changes Associated With Gill Damage in Atlantic Salmon ( Salmo salar )
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2022.806484
Figure Legend Snippet: Overview of the field trial and the microarray experimental design. (A) Abiotic and biotic stressors potentially contributing to gill damage of farmed Atlantic salmon. (B) Common reference design microarray experiment. Arrows represent microarrays with the numbers of biological replicates shown next to the arrows. The base of the arrow shows the Cy3-labeled sample (i.e., common reference pool), and the arrowhead shows the Cy5-labeled sample (i.e., experimental sample). This figure was constructed using BioRender ( https://biorender.com/ ).
Techniques Used: Microarray, Labeling, Construct
Figure Legend Snippet: Primers used in the gill either in the preliminary qPCR or the microarray validation experiment, including comparison between microarray Rank Products and qPCR fold-change results for Atlantic salmon transcripts responsive in moderately damaged gill.
Techniques Used: Microarray, Sequencing, Amplification, Binding Assay
Figure Legend Snippet: Bar plot of gill transcripts related to gill remodeling and wound healing (panel A–U ). On the lower right side, Panel (V) shows a scatterplot of log 2 fold-change from Rank Products microarray data vs . qPCR log 2 fold-change. Different letters indicate a significant difference between groups using one-way ANOVA. Asterisk (*) shows significance (p< 0.05) between GS0 and GS2 using t-test. Gene symbols followed by a dagger (†) are associated with p-values between 0.05 and 0.10 using either t-test (GS0 vs GS2; i.e., mmp19 ) or one-way ANOVA (i.e., tnnt2 ).
Techniques Used: Microarray
Figure Legend Snippet: Results of principal component analysis (PCA) plotted on two dimensions (Panel A ) for the gill differentially expressed microarray log 2 ratio (Cy5/Cy3). PC1 explained 50.72%, PC2 explained 25.49%, and PC3 explained 7.46% of the variability. Panel (B) Bar-plot of the percentage of the explained variance for each PC (dimension). Panel (C) PCA plotted on three dimensions for the gill differentially expressed microarray log 2 ratio (Cy5/Cy3) data.
Techniques Used: Microarray